Movie Gallary of D Lab in Nagoya University

HS-AFM movies (click on the images to play the movies)

ClpB(Molecura chaperone)

ClpB Oligomerization
Oligomerization process
ClpB 10uM ATP
10μM [ATP]
ClpB 1mM ATP
1mM [ATP]
ClpB 2mM ATP
2mM ATP

ClpB Subunit shuffling 1
Subunit shuffling I at 2mM [ATP]
ClpB Subunit shuffling 2
Subunit shufflingII at 2mM [ATP]

T. Uchihashi et al, Nat. Commun. 9, 2147 (2018).

Bacteriorhodopsin

bR D96N Cytoplasmic
Edge Fluctuation of Purple Membrabe (7 fps)

H. Yamashita et al, J. Struct. Biol. 167, 153-158 (2009).11).

bR D96N Cytoplasmic
CP (1 fps; x15 play)
bR D96N Lasr Intensity
CP: Laser Intensity Dependence (1 fps; x10 play)
bR D96N Extracellular
EC (1 fps; x10 play)

M. Shibata et al, Nat. Nanotechnol. 5, 208 - 212 (2010).

bR D96N Cytoplasmic
CP: Alternative green and blue light irradiation (1 fps; x20 play)

M. Shibata et al, Angew. Chem. Int. Ed. 50, 4410–4413 (2011).

CRISPR-Cas9

apo-Cas9
apo-Cas9 (4 fps; 5倍速)

Cas9-RNA
Cas9-RNA (5 fps; 5倍速)
Cas9-RNA-DNA
Cas9-RNA-DNA, +MgCl2 (5 fps; 3倍速)
po-Cas9-DNA on lipid
apo-Cas9-DNA on lipid (6.7 fps)

M. Shibata et al, Nat. Commun. 8, 1430 (2017).

po-Cas9-DNA on lipid
Cas9-RNA-DNA on lipid (6.7 fps; 2倍速)

2D Protein Crystals

 streptavidin
Streptavidin : Defect Diffusion (2 fps; 150 nm x 150 nm)

D. Yamamoto et al, Nanotechnology 19, 384009 (9 pp) (2008).

AnnexinV
Annexin V : Defect Diffusion (1 fps; 200 nm x 200 nm, x5 play)
AnnexinV
Annexin V : Defect Diffusion (1 fps; 150 nm x 150 nm, x5 play)
AnnexinV
Annexin V : Rotational diffusion (5 fps; 50 nm x 50 nm, x2 play)
AnnexinV
Annexin V : Crystal growth (1 fps; 200 nm x 200 nm, x10 play)

 Lysenin
Lysenine (2 fps; 80 nm x 80 nm, x3 play)
 Lysenin
Lysenine (3 fps; 250 nm x 250 nm, x10 play)

N. Yilmaz et al, Biophys. J. 105, 1397-1405 (2013).

TePixD
TePixD (2 fps; 120 nm x 120 nm)
 Lysenin
TePixD (2 fps; 40 nm x 410 nm)

Motor Proteins

F1 ATPase
Rototorless F1 ATPase
F1 ATPase
Rototorless F1 ATPase

T. Uchihashi et al, Science 333, 755-758 (2011).

Cellulase
Cellulase

K. Igarashhi et al, Science 333, 1279-1282 (2011).

Dynein
Cytoplasmic Dynein (2 fps; 200 nm x 200 nm, x5 play)

Interdomain flip-flop motion of Cellobiose dehydrogenase

CDH
CDH (50 μM cellobiose)
CDH
CDH (100 μM cellobiose)
CDH
CDH (cellobiose washed)

H. Harada et al, Chem. Sci. 9, 6561-6565 (2017).

Microtubule

Microtuble Polymerization
Polymerization (1 fps; 250 nm x 250 nm, x10 play)
Microtuble Polymerization
Destruction and Repolymerization (3.3 fps; 100 nm x 60 nm, x3 play)
HR Microtuble
High-Resolution Imaging
HR Microtuble
High-Resolution Imaging

Bacteria

Bacilus-Lysozyme
Bacteriolysis of Bacillus subtilis induced by Lysozyme (0.05 fps; 5μm x 5μm, x100 play)
Bacilus-Lysozyme
Bacteriolysis (0.5 fps; 500 nm x 500 nm, x10 play)

H. Watanabe et al, Rev. Sci. Instrum. 84, 053702 (2013).

Magnetic Bacteria
Surface structure of Magnetosomes
Magnetic Bacteria
Surface structure of Magnetosomes

H. Yamashita et al, J. Mol. Biol. 422(2), 300-309 (2012).

Living Cell

Cos-7 actin flow
COS-7, Actine Retrograde Flow
Cos-7 inslulin
COS-7, Activation by Insulin
Cos-7 pit formation
COS-7, Pit Formation
Neuron
Nerve Cell

M. Shibata et al, Sci. Rep. 5, 8724 (7 pp) (2015).

Non-biological samples

Detergent
Adsorption process of cationic detergent(CTAC) to mica substrate
Rinse
Adsorption process of rinse to a huma hair
  
Smapoo
Washing of a human hair by shampoo
  

S. Inoue et al, Chem Comm. 47, 4974-4976 (2011).


Homopolymer Al–An
Homopolymer Al–An on HOPG in methylcyclohexane
Homopolymer Al–An
Flexibility of homopolymer Al–An
  

M. Hosoyamada et al, Chem Comm. 54, 6828-6831 (2018).