Papers

2019

  1. Wataru Shihoya, Keiichi Inoue, Manish Singh, Masae Konno, Shoko Hososhima, Keitaro Yamashita, Kento Ikeda, Akimitsu Higuchi, Tamaki Izume, Sae Okazaki, Masanori Hashimoto, Ritsu Mizutori, Sahoko Tomida, Yumeka Yamauchi, Rei Abe-Yoshizumi, Kota Katayama, Satoshi P. Tsunoda, Mikihiro Shibata, Yuji Furutani, Alina Pushkarev, Oded Béjà, Takayuki Uchihashi, Hideki Kandori* and Osamu Nureki*, "Crystal structure of heliorhodopsin, Nature, published online. DOI: 10.1038/s41586-019-1604-6
  2. Rina Yogo, Yuki Yamaguchi, Hiroki Watanabe, Hirokazu Yagi, Tadashi Satoh, Mahito Nakanishi, Masayoshi Onitsuka, Takeshi Omasa, Mari Shimada, Takahiro Maruno, Tetsuo Torisu, Shio Watanabe, Daisuke Higo, Takayuki Uchihashi, Saeko Yanaka, Susumu Uchiyama and Koichi Kato*, "The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III", Scientific Reports 9 , 11957 (2019).DOI: 10.1038/s41598-019-48323-w
  3. Shusuke Matsui, Kensuke Hosho, Haruka Minato, Takayuki Uchihashi* and Daisuke Suzuki*, "Protein Uptake into Individual Hydrogel Microspheres Visualized by High-Speed Atomic Force Microscopy", Chemical Communications 55, 10064-10067 (2019). DOI: 10.1039/C9CC05116C
  4. Lei Feng, Hiroki Wantanabe, Paul Molino, Gordon Wallace, Son Lam Phung, Takayuki Uchihashi and Michael Higgins*, "Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy", Journal of Molecular Biology 46, 2687-2699 (2019). DOI: 10.1016/j.jmb.2019.04.044
  5. Taichiro Sekiguchi, Tadashi Satoh, Eiji Kurimoto, Chihong Son, Toshiya Kozai, Hiroki Watanabe, Kentaro Ishii, Hirokazu Yagi, Saeko Yanaka Susumu Uchiyama, Takayuki Uchihashi, Kazuyoshi Murata and Koichi Kato*, "Mutational and combinatorial control of self-assembling and disassembling of human proteasome α-subunits", International Journal of Molecular Sciences 20, 2608 (2019). DOI: 0.3390/ijms20092308.
  6. Yuichiro Nishizawa, Shusuke Matsui, Kenji Urayama, Takuma Kureha, Mitsuhiro Shibayama, Takayuki Uchihashi* and Daisuke Suzuki*, "Non-Thermoresponsive Decanano-sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature-Controlled High-Speed Atomic Force Microscopy", Angewandte Chemie International Edition 58, 1-6 (2019). DOI:10.1002/anie.201903483.
  7. Ayumi Sumino#*, Takashi Sumikama#, Takayuki Uchihashi*, Shigetoshi Oiki*, "High-speed AFM reveals accelerated binding of Agitoxin-2 to K+ channel by induced-fit", Science Advances 5, eaax0495 (2019). DOI: 10.1126/sciadv.aax0495 (#equal contribution)
  8. Takaaki Miyamoto, Yugo Hayashi, Keito Yoshida, Hiroki Watanabe, Takayuki Uchihashi, Kento Yonezawa, Nobutaka Shimizu, Hironari Kamikubo, Shun Hirota*, "Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins", ACS Synthetic Biology 8, 1112-1120 (2019). DOI: 10.1021/acssynbio.9b00019.
  9. Kenshiro Honda, Yuka Sazuka, Kojiro Iizuka, Shusuke Matsui, Takayuki Uchihashi, Takuma Kureha, Mitsuhiro Shibayama, Takumi Watanabe and Daisuke Suzuki*, "Hydrogel Microellipsoids that Form Robust String-like Assemblies at the Air/Water Interface", Angewandte Chemie International Edition 58, 1-6 (2019). DOI:10.1002/anie.201901611.
  10. Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi, Toshio Ando*, "High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies", Biochimica et Biophysica Acta (BBA) - General Subjects. (in press)
  11. Elena Krayukhina, Masami Yokoyama, Kayoko Kakuhou Hayashihara, Takahiro Maruno, Masanori Noda, Hiroki Watanabe, Takayuki Uchihashi, Susumu Uchiyama*, "An assessment of the ability of submicron- and micron-size silicone oil droplets indropped pre-fillable syringes to invoke early- and late-stage immune responses", Journal of Pharmaceutical Sciences 108, 2278-2287 (2019). DOI: 10.1016/j.xphs.2019.02.002
  12. Mikito Owa, Takayuki Uchihashi, Haru-aki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando and Masahide Kikkawa*, "Inner lumen proteins stabilize doublet microtubules in cilia and flagella", Nature Communications 10, Article number: 1143 (2019). DOI: 10.1038/s41467-019-09051-x.
  13. Shintaro Maruyama, Kano Suzuki,Motonori Imamura, Hikaru Sasaki, Hideyuki Matsunami, Kenji Mizutani, Yasuko Saito, Fabiana L. Imai, Yoshiko Ishizuka-Katsura, Tomomi Kimura-Someya, Mikako Shirouzu, Takayuki Uchihashi, Toshio Ando, Ichiro Yamato and Takeshi Murata*, "Metastable asymmetrical structure of a shaftless V1 motor", Science Advances 5, eaau8149 (2019). DOI: 10.1126/sciadv.aau8149.
  14. Koji Oohora*, Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi* and Takashi Hayashi*, "A Ring-shaped Hemoprotein Trimer Thermodynamically Controlled by the Supramolecular Heme–Heme Pocket Interaction", Chemical Communications 55, 1544-1547 (2019). DOI: 10.1039/C8CC09314H.
  15. Christian Ganser* and Takayuki Uchihashi*, "Microtubule self-healing and defect creation investigated by in-line force measurements during high-speed atomic force microscopy", Nanoscale 11, 125-135 (2019). DOI: 10.1039/C8NR07392A.
  16. Takamitsu Haruyama#, Yasunori Sugano#, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Yoshiki Tanaka, Hiroki Konno*, and Tomoya Tsukazaki*, "Single-unit imaging of membrane protein-embedded nanodiscs from two oriented sides by high-speed atomic force microscopy", Structure 27, 152-160(2019). DOI: 10.1016/j.str.2018.09.005. (#equal contribution)

2018

  1. Tomoya Fukui, Takayuki Uchihashi*,Norihiko Sasaki, Hiroki Watanabe, Masayuki Takeuchi* and Kazunori Sugiyasu*, "Direct observation and manipulation of supramolecular polymerization by using high-speed atomic force microscopy", Angewandte Chemie International Edition 57, 15465-15470 (2018). DOI: 10.1002/anie.201809165.
  2. Shusuke Matsui, Yuichiro Nishizawa, Takayuki Uchihashi* and Daisuke Suzuki*, "Monitoring Thermo-responsive Morphological Changes in Individual Hydrogel Microspheres", ACS Omega 3, 10836-10842 (2018). DOI: 10.1021/acsomega.8b01770.
  3. Koji Oohora*, Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi* and Takashi Hayashi*, "Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling", Journal of American Chemical Society 140, 10145-10148 (2018). DOI: 10.1021/jacs.8b06690.
  4. Tetsuya Mori#, Shogo Sugiyama#, Mark Byne, Carl Hirschie Johnson*, Takayuki Uchihashi* and Toshio Ando*, "Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time", Nature Communications 9, Article number: 3245 (2018). DOI: 10.1038/s41467-018-05438-4. (#equal contribution)
  5. Takayuki Uchihashi*, Hiroki Watanabe and Noriyuki Kodera, "Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy", Methods in Molecular Biology 1814, 159-179 (2018). DOI: 10.1007/978-1-4939-8591-3_10.
  6. Masanori Hosoyamada, Nobuhiro Yanai*, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka*, "Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion", Chemical Communications 54, 6828-6831 (2018) DOI: 10.1039/c8cc01594e.
  7. Takayuki Uchihashi#, Yo-hei Watanabe#*, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino*, Toshio Ando*, "Dynamic structural states of ClpB involved in its disaggregation function", Nature Communications 9, Article number: 2147 (2018). DOI: 10.1038/s41467-018-04587-w. (#equal contribution)
  8. Mikihiro Shibata#, Keiichi Inoue#, Kento Ikeda, Masae Konno, Manish Singh, Chihiro Kataoka, Rei Abe-Yoshizumi, Hideki Kandori*, Takayuki Uchihashi*, "Oligomeric states of microbial rhodopsins determined by high-speed atomic force microscopy and circular dichroic spectroscopy", Scientific Reports 8, Article number: 8262 (2018). DOI: 110.1038/s41598-018-26606-y.(#equal contribution)
  9. Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama*, "Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation", Journal of Pharmaceutical Sciences 107, pp. 1521-1529 (2018). DOI: 10.1016/j.xphs.2018.01.021.
  10. Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda*, "Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy", Colloids and Surfaces B: Biointerfaces 167, pp. 264-274 (2018). DOI: 10.1016/j.colsurfb.2018.04.015.
  11. Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino*, "Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export", Science Advances 4, eaao7054 (2018). DOI: 10.1126/sciadv.aao7054.
  12. Hisao Tsukamoto, Masahiro Higashi, Hideyoshi Motoki, Hiroki Watanabe, Christian Ganser, Koichi Nakajo, Yoshihiro Kubo, Takayuki Uchihashi and Yuji Furutani*, "Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel", Journal of Biological Chemistry 293, pp. 6969-6984 (2018). DOI: 10.1074/jbc.RA118.001817.
  13. Akiya Oda, Satoshi Nagao, Masaru Yamanaka, Hiroki Watanabe, Takayuki Uchihashi, Ikki Ueda, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota*, "Construction of a Triangle-Shaped Trimer and a Tetrahedral Structure Using an α-Helix-Inserted Circular Permutant of Cytochrome c555", Chemistry - An Asian Journal 13, pp. 964-967 (2018). DOI: 10.1002/asia.201800252.
  14. Takamitsu Haruyama, Takayuki Uchihashi, Yutaro Yamada, Noriyuki Kodera, Toshio Ando and Hiroki Konno*, "Negatively charged lipids are essential for functional and structural switch of human 2-Cys peroxiredoxin II", Journal of Molecular Biology 430, pp. 602-610 (2018). DOI: 10.1016/j.jmb.2017.12.020.
  15. Tetsuya Takeda*, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando* and Kohji Takei*, "Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis", e-Life 7, e3024 (2018). DOI: 10.7554/eLife.30246.
  16. Maho Yagi-Utsumi, Arunima Sikdar, Toshiya Kozai, Rintaro Inoue, Masaaki Sugiyama, Takayuki Uchihashi, Tadashi Satoh and Koichi Kato*, "Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation", Protein engineering design & selection 31, 29-36 (2018). DOI: 10.1093/protein/gzx066.
  17. Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino*, "Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis", Physical Chemistry Chemical Physics 20, 3010-3018 (2018). DOI: 10.1039/C7CP04606E.
  18. Takayuki Uchihashi* and Simon Scheuring, "Review: Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes", BBA General Subjects 1862, 229-240 (2018). DOI: 10.1016/j.bbagen.2017.07.010.

2017

  1. Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato* and Takayuki Uchihashi*, "Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy", Scientific Reports 7, article number: 15373 (2017). DOI: 10.1038/s41598-017-15708-8.
  2. Naoya Terahara, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Keiichi Namba* and Tohru Minamino*, "Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor", Science Advances 3, eaao4119 (2017). DOI: 10.1126/sciadv.aao4119.
  3. Mikihiro Shibata*, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda, "High-speed atomic force microscopy imaging of live mammalian cells", Biophysics and Physicobiology 14, 127-135 (2017). DOI: 10.2142/biophysico.14.0_127
  4. Mikihiro Shibata*, Hiroshi Nishimasu*, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Takayuki Uchihashi and Osamu Nureki*, "Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy", Nature Communications 8, article number: 1430 (2017). DOI: 10.1038/s41467-017-01466-8.
  5. Tadashi Satoh*, Chihong Song, Tong Zhu, Takayasu Toshimori, Kazuyoshi Murata, Yugo Hayashi, Hironari Kamikubo, Takayuki Uchihashi and Koichi Kato*, "Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT", Scientific Reports 7, Article Number: 12142 (2017). DOI: 10.1038/s41598-017-12283-w.
  6. Hirofumi Harada, Akira Onoda*, Takayuki Uchihashi*, Hiroki Watanabe, Naoki Sunagawa, Masahiro Samejima, Kiyohiko Igarashi*, and Takashi Hayashi, "Interdomain Flip-flop Motion Visualized in Flavocytochrome Cellobiose Dehydrogenase Using High-speed Atomic Force Microscopy during Catalysis", Chemical Science 8, 6561-6565 (2017). DOI: 10.1039/c7sc01672g.
  7. Shusuke Matsui, Takuma Kureha, Seina Hiroshige, Mikihiro Shibata,Takayuki Uchihashi*, and Daisuke Suzuki*, "Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution", Angewandte Chemie International Edition (Communication) 56, 12146-12149 (2017). DOI: 10.1002/anie.201705808.
  8. Jakia Jannat Keya, Daisuke Inoue, Yuki Suzuki, Toshiya Kozai, Daiki Ishikuro, Noriyuki Kodera, Takayuki Uchihashi, Arif Md. Rashedul Kabir, Masayuki Endo, Kazuki Sada, Akira Kakugo, "High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM", Scientific Reports 7, 6166 (2017). DOI: 10.1038/s41598-017-06249-1.
  9. Mahmoud Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando and Richard Wong*, "High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells", ACS Nano 11, 5567-5578 (2017). DOI: 10.1021/acsnano.7b00906.
  10. Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki*, "Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging ", J. Phy. Chem. Lett. 8, 785-793 (2017). DOI: 10.1021/acs.jpclett.6b03058.