論文

4

2019

  1. Lei Feng, Hiroki Wantanabe, Paul Molino, Gordon Wallace, Son Lam Phung, Takayuki Uchihashi and Michael Higgins*, "Dynamics of Inter-Molecular Interactions Between Single Aβ42 Oligomeric and Aggregate Species by High-Speed Atomic Force Microscopy", Journal of Molecular Biology46, 2687-2699 (2019). DOI: 10.1016/j.jmb.2019.04.044
  2. Taichiro Sekiguchi, Tadashi Satoh, Eiji Kurimoto, Chihong Son6, Toshiya Kozai, Hiroki Watanabe, Kentaro Ishii, Hirokazu Yagi, Saeko Yanaka Susumu Uchiyama, Takayuki Uchihashi, Kazuyoshi Murata and Koichi Kato*, "Mutational and combinatorial control of self-assembling and disassembling of human proteasome α-subunits", International Journal of Molecular Sciences 20, 2608 (2019). DOI: 0.3390/ijms20092308.
  3. Yuichiro Nishizawa, Shusuke Matsui, Kenji Urayama, Takuma Kureha, Mitsuhiro Shibayama, Takayuki Uchihashi* and Daisuke Suzuki*, "Non-Thermoresponsive Decanano-sized Domains in Thermoresponsive Hydrogel Microspheres Revealed by Temperature-Controlled High-Speed Atomic Force Microscopy", Angewandte Chemie International Edition58, 1-6 (2019). DOI:10.1002/anie.201903483.
  4. Ayumi Sumino#*, Takashi Sumikama#, Takayuki Uchihashi*, Shigetoshi Oiki*, "High-speed AFM reveals accelerated binding of Agitoxin-2 to K+ channel by induced-fit", Science Advances. (accepted)(#equal contribution)
  5. Takaaki Miyamoto, Yugo Hayashi, Keito Yoshida, Hiroki Watanabe, Takayuki Uchihashi, Kento Yonezawa, Nobutaka Shimizu, Hironari Kamikubo, Shun Hirota*, "Construction of a quadrangular tetramer and a cage-like hexamer from three-helix bundle-linked fusion proteins", ACS Synthetic Biology 8, 1112-1120 (2019). DOI: 10.1021/acssynbio.9b00019.
  6. Kenshiro Honda, Yuka Sazuka, Kojiro Iizuka, Shusuke Matsui, Takayuki Uchihashi, Takuma Kureha, Mitsuhiro Shibayama, Takumi Watanabe and Daisuke Suzuki*, "Hydrogel Microellipsoids that Form Robust String-like Assemblies at the Air/Water Interface", Angewandte Chemie International Edition 58, 1-6 (2019). DOI:10.1002/anie.201901611.
  7. Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi, Toshio Ando*, "High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies", Biochimica et Biophysica Acta (BBA) - General Subjects. (in press)
  8. Elena Krayukhina, Masami Yokoyama, Kayoko Kakuhou Hayashihara, Takahiro Maruno, Masanori Noda, Hiroki Watanabe, Takayuki Uchihashi, Susumu Uchiyama*, "An assessment of the ability of submicron- and micron-size silicone oil droplets indropped pre-fillable syringes to invoke early- and late-stage immune responses", Journal of Pharmaceutical Sciences. 108, 2278-2287 (2019). DOI: 10.1016/j.xphs.2019.02.002
  9. Mikito Owa, Takayuki Uchihashi, Haru-aki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando and Masahide Kikkawa*, "Inner lumen proteins stabilize doublet microtubules in cilia and flagella", Nature Communications 10, Article number: 1143 (2019). DOI: 10.1038/s41467-019-09051-x.
  10. Shintaro Maruyama, Kano Suzuki,Motonori Imamura, Hikaru Sasaki, Hideyuki Matsunami, Kenji Mizutani, Yasuko Saito, Fabiana L. Imai, Yoshiko Ishizuka-Katsura, Tomomi Kimura-Someya, Mikako Shirouzu, Takayuki Uchihashi, Toshio Ando, Ichiro Yamato and Takeshi Murata*, "Metastable asymmetrical structure of a shaftless V1 motor", Science Advances 5, eaau8149 (2019). DOI: 10.1126/sciadv.aau8149.
  11. Koji Oohora*, Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi* and Takashi Hayashi*, "A Ring-shaped Hemoprotein Trimer Thermodynamically Controlled by the Supramolecular Heme–Heme Pocket Interaction", Chemical Communications 55, 1544-1547 (2019). DOI: 10.1039/C8CC09314H.
  12. Christian Ganser* and Takayuki Uchihashi*, "Microtubule self-healing and defect creation investigated by in-line force measurements during high-speed atomic force microscopy", Nanoscale 11, 125-135 (2019). DOI: 10.1039/C8NR07392A.
  13. Takamitsu Haruyama#, Yasunori Sugano#, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Yoshiki Tanaka, Hiroki Konno*, and Tomoya Tsukazaki*, "Single-unit imaging of membrane protein-embedded nanodiscs from two oriented sides by high-speed atomic force microscopy", Structure 27, 152-160(2019). DOI: 10.1016/j.str.2018.09.005. (#equal contribution)

2018

  1. Tomoya Fukui, Takayuki Uchihashi*,Norihiko Sasaki, Hiroki Watanabe, Masayuki Takeuchi* and Kazunori Sugiyasu*, "Direct observation and manipulation of supramolecular polymerization by using high-speed atomic force microscopy", Angewandte Chemie International Edition 57, 15465-15470 (2018). DOI: 10.1002/anie.201809165.
  2. Shusuke Matsui, Yuichiro Nishizawa, Takayuki Uchihashi* and Daisuke Suzuki*, "Monitoring Thermo-responsive Morphological Changes in Individual Hydrogel Microspheres", ACS Omega 3, 10836-10842 (2018). DOI: 10.1021/acsomega.8b01770.
  3. Koji Oohora*, Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi* and Takashi Hayashi*, "Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling", Journal of American Chemical Society 140, 10145-10148 (2018). DOI: 10.1021/jacs.8b06690.
  4. Tetsuya Mori#, Shogo Sugiyama#, Mark Byne, Carl Hirschie Johnson*, Takayuki Uchihashi* and Toshio Ando*, "Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time", Nature Communications 9, Article number: 3245 (2018). DOI: 10.1038/s41467-018-05438-4. (#equal contribution)
  5. Takayuki Uchihashi*, Hiroki Watanabe and Noriyuki Kodera, "Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy", Methods in Molecular Biology 1814, 159-179 (2018). DOI: 10.1007/978-1-4939-8591-3_10.
  6. Masanori Hosoyamada, Nobuhiro Yanai*, Keisuke Okumura, Takayuki Uchihashi and Nobuo Kimizuka*, "Translating MOF chemistry into supramolecular chemistry: soluble coordination nanofibers showing efficient photon upconversion", Chemical Communications 54, 6828-6831 (2018) DOI: 10.1039/c8cc01594e.
  7. Takayuki Uchihashi#, Yo-hei Watanabe#*, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino*, Toshio Ando*, "Dynamic structural states of ClpB involved in its disaggregation function", Nature Communications 9, Article number: 2147 (2018). DOI: 10.1038/s41467-018-04587-w. (#equal contribution)
  8. Mikihiro Shibata#, Keiichi Inoue#, Kento Ikeda, Masae Konno, Manish Singh, Chihiro Kataoka, Rei Abe-Yoshizumi, Hideki Kandori*, Takayuki Uchihashi*, "Oligomeric states of microbial rhodopsins determined by high-speed atomic force microscopy and circular dichroic spectroscopy", Scientific Reports 8, Article number: 8262 (2018). DOI: 110.1038/s41598-018-26606-y.(#equal contribution)
  9. Takahiro Maruno, Hiroki Watanabe, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi and Susumu Uchiyama*, "Sweeping of adsorbed therapeutic proteins on prefillable syringe enhances subvisible particles generation", Journal of Pharmaceutical Sciences 107, pp. 1521-1529 (2018). DOI: 10.1016/j.xphs.2018.01.021.
  10. Takayuki Umakoshi, Hikari Udaka, Takayuki Uchihashi, Toshio Ando, Miho Suzuki, Takeshi Fukuda*, "Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed atomic force microscopy", Colloids and Surfaces B: Biointerfaces 167, pp. 264-274 (2018). DOI: 10.1016/j.colsurfb.2018.04.015.
  11. Naoya Terahara, Yumi Inoue, Noriyuki Kodera, Yusuke V. Morimoto, Takayuki Uchihashi, Katsumi Imada, Toshio Ando, Keiichi Namba, and Thoru Minamino*, "Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export", Science Advances 4, eaao7054 (2018). DOI: 10.1126/sciadv.aao7054.
  12. Hisao Tsukamoto, Masahiro Higashi, Hideyoshi Motoki, Hiroki Watanabe, Christian Ganser, Koichi Nakajo, Yoshihiro Kubo, Takayuki Uchihashi and Yuji Furutani*, "Structural properties determining low K+ affinity of the selectivity filter in the TWIK1 K+ channel", Journal of Biological Chemistry 293, pp. 6969-6984 (2018). DOI: 10.1074/jbc.RA118.001817.
  13. Akiya Oda, Satoshi Nagao, Masaru Yamanaka, Hiroki Watanabe, Takayuki Uchihashi, Ikki Ueda, Naoki Shibata, Yoshiki Higuchi, and Shun Hirota*, "Construction of a Triangle-Shaped Trimer and a Tetrahedral Structure Using an α-Helix-Inserted Circular Permutant of Cytochrome c555", Chemistry - An Asian Journal 13, pp. 964-967 (2018). DOI: 10.1002/asia.201800252.
  14. Takamitsu Haruyama, Takayuki Uchihashi, Yutaro Yamada, Noriyuki Kodera, Toshio Ando and Hiroki Konno*, "Negatively charged lipids are essential for functional and structural switch of human 2-Cys peroxiredoxin II", Journal of Molecular Biology 430, pp. 602-610 (2018). DOI: 10.1016/j.jmb.2017.12.020.
  15. Tetsuya Takeda*, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando* and Kohji Takei*, "Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis", e-Life 7, e3024 (2018). DOI: 10.7554/eLife.30246.
  16. Maho Yagi-Utsumi, Arunima Sikdar, Toshiya Kozai, Rintaro Inoue, Masaaki Sugiyama, Takayuki Uchihashi, Tadashi Satoh and Koichi Kato*, "Conversion of functionally undefined homopentameric protein PbaA into a proteasome activator by mutational modification of its C-terminal segment conformation", Protein engineering design & selection 31, 29-36 (2018). DOI: 10.1039/C7CP04606E.
  17. Akihiko Nakamura, Tomoyuki Tasaki, Yasuko Okuni, Chihong Song, Kazuyoshi Murata, Toshiya Kozai, Mayu Hara, Hayuki Sugimoto, Kazushi Suzuki, Takeshi Watanabe, Takayuki Uchihashi, Hiroyuki Noji and Ryota Iino*, "Rate constants, processivity, and productive binding ratio of chitinase A revealed by single-molecule analysis", Physical Chemistry Chemical Physics 20, 3010-3018 (2018). DOI: 10.1093/protein/gzx066.
  18. Takayuki Uchihashi* and Simon Scheuring, "Review: Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes", BBA General Subjects 1862, 229-240 (2018). DOI: 10.1016/j.bbagen.2017.07.010.

2017

  1. Toshiya Kozai, Taichiro Sekiguchi, Tadashi Satoh, Hirokazu Yagi, Koichi Kato* and Takayuki Uchihashi*, "Two-step process for disassembly mechanism of proteasome α7 homo-tetradecamer by α6 revealed by high-speed atomic force microscopy", Scientific Reports 7, article number: 15373 (2017). DOI: 10.1038/s41598-017-15708-8.
  2. Naoya Terahara, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Keiichi Namba* and Tohru Minamino*, "Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor", Science Advances 3, eaao4119 (2017). DOI: 10.1126/sciadv.aao4119.
  3. Mikihiro Shibata*, Hiroki Watanabe, Takayuki Uchihashi, Toshio Ando, and Ryohei Yasuda, "High-speed atomic force microscopy imaging of live mammalian cells", Biophysics and Physicobiology 14, 127-135 (2017). DOI: 10.2142/biophysico.14.0_127.
  4. Mikihiro Shibata*, Hiroshi Nishimasu*, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Takayuki Uchihashi and Osamu Nureki*, "Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy", Nature Communications 8, article number: 1430 (2017). DOI: 10.1038/s41467-017-01466-8.
  5. Tadashi Satoh*, Chihong Song, Tong Zhu, Takayasu Toshimori, Kazuyoshi Murata, Yugo Hayashi, Hironari Kamikubo, Takayuki Uchihashi and Koichi Kato*, "Visualisation of a flexible modular structure of the ER folding-sensor enzyme UGGT", Scientific Reports 7, Article Number: 12142 (2017). DOI: 10.1038/s41598-017-12283-w.
  6. Hirofumi Harada, Akira Onoda*, Takayuki Uchihashi*, Hiroki Watanabe, Naoki Sunagawa, Masahiro Samejima, Kiyohiko Igarashi*, and Takashi Hayashi, "Interdomain Flip-flop Motion Visualized in Flavocytochrome Cellobiose Dehydrogenase Using High-speed Atomic Force Microscopy during Catalysis", Chemical Science 8, 6561-6565 (2017). DOI: 10.1039/c7sc01672g.
  7. Shusuke Matsui, Takuma Kureha, Seina Hiroshige, Mikihiro Shibata,Takayuki Uchihashi*, and Daisuke Suzuki*, "Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution", Angewandte Chemie International Edition (Communication) 56, 12146-12149 (2017). DOI: 10.1002/anie.201705808.
  8. Jakia Jannat Keya, Daisuke Inoue, Yuki Suzuki, Toshiya Kozai, Daiki Ishikuro, Noriyuki Kodera, Takayuki Uchihashi, Arif Md. Rashedul Kabir, Masayuki Endo, Kazuki Sada, Akira Kakugo, "High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM", Scientific Reports 7, 6166 (2017). DOI: 10.1038/s41598-017-06249-1.
  9. Mahmoud Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando and Richard Wong*, "High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells", ACS Nano 11, 5567-5578 (2017). DOI: 10.1021/acsnano.7b00906.
  10. Ayumi Sumino, Takayuki Uchihashi and Shigetoshi Oiki*, "Oriented Reconstitution of the Full-Length KcsA Potassium Channel in a Lipid Bilayer for AFM Imaging ", J. Phy. Chem. Lett. 8, 785-793 (2017). DOI: 10.1021/acs.jpclett.6b03058.